Name : Mouse TFPI-2 Protein
Product Source :
Recombinant Mouse TFPI-2 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Leu23-Ser230.[Accession | O35536]
Molecular Weight :
The protein has a predicted MW of 24.87 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse TFPI-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Background :
Tissue factor pathway inhibitor-2 (TFPI-2) has previously been characterized as an endogenous anticoagulant. TFPI-2 is expressed in the vast majority of cells, mainly secreted into the extracellular matrix. Moreover, in sputum from cystic fibrosis patients TFPI-2 C-terminal fragments are generated and found associated with immunoglobulins. Together our data describe a previously unknown host defense mechanism and therapeutic importance of TFPI-2 against invading Gram-negative bacterial pathogens.
Synonyms :
TFPI-2; PP5; TFPI2; REF1; TFPI-2REF1
References & Citations :
(1) Ali MN, Kasetty G, Elvén M, Alyafei S, Jovic S, Egesten A, Herwald H, Schmidtchen A, Papareddy P. TFPI-2 Protects Against Gram-Negative Bacterial Infection. Front Immunol. 2018 Sep 11;9:2072. doi: 10.3389/fimmu.2018.02072. PMID: 30254643; PMCID: PMC6141739.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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