Name : Biotinylated Human Mature TGF beta 3 Protein

Product Source :
Recombinant Biotinylated Human Mature TGF beta 3 Protein is expressed from HEK293 with Avi tag at the C-Terminus. It contains Ala301-Ser412.[Accession | P10600-1]

Molecular Weight :
The protein has a predicted MW of 14.53 kDa. Due to glycosylation, the protein migrates to 15-20 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22μm filtered solution in 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCl.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Biotinylated Human Mature TGF beta 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human TGF-beta RII, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 3, Avi Tag with the EC50 of 6.4ng/ml determined by ELISA.

Background :
Transforming growth factor-beta 3 is a cytokine which is involved in cell growth regulation and differentiation, stimulation of extracellular matrix and modulation of immune responses. TGF beta 3 RNA is expressed in a diverse array of tissues including perichondrium, bone, intervertebral discs, mesenteries, pleura, heart, lung, palate, and amnion, as well as in central nervous system (CNS) structures such as the meninges, choroid plexus and the olfactory bulbs. Furthermore, in several organ systems, TGF beta 3 transcripts are expressed during periods of active morphogenesis suggesting that the protein may be an important factor for the growth and differentiation of many embryonic tissues.

Synonyms :
TGF-beta-3; LAP; TGFB3; ARVD; ARVD1; RNHF;TGF-β-3

References & Citations :
(1)Kuşcu NK, Laçin S, Vatansever S, Yildirim Y, Var A, Uyanik BS, Koyuncu F. Immunolocalization of transforming growth factor-beta 3 in pregnant human myometrium. Acta Obstet Gynecol Scand. 2001 Dec;80(12):1079-83. PMID: 11846702.(2)Pelton RW, Dickinson ME, Moses HL, Hogan BL. In situ hybridization analysis of TGF beta 3 RNA expression during mouse development: comparative studies with TGF beta 1 and beta 2. Development. 1990 Oct;110(2):609-20. doi: 10.1242/dev.110.2.609. PMID: 1723948.

MedChemExpress (MCE) recombinant proteins include: cytokines, enzymes, growth factors, hormones, receptors, transcription factors, antibody fragments, etc. They are often essential for supporting cell growth, stimulating cell signaling pathways, triggering or inhibiting cell differentiation; and are useful tools for elucidating protein structure and function, understanding disease onset and progression, and validating pharmaceutical targets. At MedChemExpress (MCE), we strive to provide products with only the highest quality. Protein identity, purity and biological activity are assured by our robust quality control and assurance procedures.
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