Name : Human Latent TGF beta 2/TGFB2 Protein
Product Source :
Recombinant Human Latent TGF beta 2/TGFB2 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Leu21-Ser414.[Accession | P61812-1]
Molecular Weight :
The protein has a predicted MW of 46.6 kDa. Due to glycosylation, the protein migrates to 40-60 kDa&12-13 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Latent TGF beta 2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Latent TGF beta 2 is greater than 95% as determined by SEC-HPLC. SPR Data Human TGF-beta RII, hFc Tag captured on CM5 Chip via Protein A can bind Human Latent TGF beta 2, His Tag with an affinity constant of 2.21 μM as determined in SPR assay (Biacore T200).
Background :
Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.
Synonyms :
TGFB2; TGFBR2; TGFR2; TbetaR-II; TGFβR2; BB105277; TGF-beta 2; TβR-II; TGF-β 2
References & Citations :
Yao K, Tan J, Gu WZ, Ye PP, Wang KJ. Reactive oxygen species mediates the apoptosis induced by transforming growth factor beta(2) in human lens epithelial cells. Biochem Biophys Res Commun. 2007 Mar 2;354(1):278-83. doi: 10.1016/j.bbrc.2006.12.198. Epub 2007 Jan 10. PMID: 17217916.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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