Name : Human Latent TGF beta 3 Protein
Product Source :
Recombinant Human Latent TGF beta 3 Protein is expressed from HEK293 with His tag at the N-Terminus. It contains Leu24-Ser412.[Accession | P10600-1]
Molecular Weight :
The protein has a predicted MW of 45.9 kDa. Due to glycosylation, the protein migrates to 46-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Latent TGF beta 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human Latent TGF beta 3, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 14.7ng/ml determined by ELISA.
Background :
Transforming growth factor-betas (TGF-βs) are multifunctional cytokines that have been implicated in the regulation of a broad range of biological processes, including cell proliferation, cell survival, and cell differentiation. And transforming growth factor beta3 (TGFbeta3) is a key protein involved in scar-free healing observed in embryos.
Synonyms :
TGFB3; ARVD; TGF-beta3; TGF-β; TGFβ3; TGFβ
References & Citations :
(1) Okamura T, Morita K, Iwasaki Y, Inoue M, Komai T, Fujio K, Yamamoto K. Role of TGF-β3 in the regulation of immune responses. Clin Exp Rheumatol. 2015 Jul-Aug;33(4 Suppl 92):S63-9. Epub 2015 Oct 12. PMID: 26457612.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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