Name : Human TGFBR1 Protein
Product Source :
Recombinant Human TGFBR1 Protein is expressed from HEK293 with mFc (IgG1) tag and Avi tag at the C-Terminus. It contains Leu34-Glu125.[Accession | P36897-1]
Molecular Weight :
The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TGFBR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TGFBR1 is greater than 95% as determined by SEC-HPLC. SPR Data Human TGFBR1, mFc Tag captured on CM5 Chip via Protein A can bind Human Mature TGF beta 1, No Tag with an affinity constant of 0.35 μM as determined in SPR assay (Biacore T200).
Background :
transforming growth factor beta receptor 1 (TGFBR1), a key stimulator of tumor proliferation and metastasis, was a direct target of miR‑98‑5p. miR‑98‑5p overexpression resulted in the downregulation of TGFBR1 and the suppression of the viability, proliferation, migration and invasion of A549 and H1299 cells.
Synonyms :
AAT5; ACVRLK4; ALK-5; LDS1A; LDS2A; SKR4; tbetaR-I; TGFB1R1; TGF-beta RI; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; tβR-I; TGF-β RI; TGFβRI
References & Citations :
(1)Jiang F, Yu Q, Chu Y, Zhu X, Lu W, Liu Q, Wang Q. MicroRNA-98-5p inhibits proliferation and metastasis in non-small cell lung cancer by targeting TGFBR1. Int J Oncol. 2019 Jan;54(1):128-138. doi: 10.3892/ijo.2018.4610. Epub 2018 Oct 29. PMID: 30387848; PMCID: PMC6255066.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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