Name : Cynomolgus/Rhesus macaque TIGIT Protein
Product Source :
Recombinant Cynomolgus/Rhesus macaque TIGIT Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Met22-Ile141.[Accession | XP_015300911.1]
Molecular Weight :
The protein has a predicted MW of 14.4 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus/Rhesus macaque TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus/Rhesus macaque TIGIT is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus/Rhesus macaque TIGIT, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TIGIT Antibody, hFc Tag with the EC50 of 14.1ng/ml determined by ELISA.
Background :
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.IGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Synonyms :
TIGIT; VSIG9; VSTM3; WUCAM
References & Citations :
(1)Yu X , Harden K , C Gonzalez L , et al. The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells[J]. NATURE IMMUNOLOGY, 2008, 10(1):48-57.
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