Name : Human TIMP-2 Protein
Product Source :
Recombinant Human TIMP-2 Protein Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Cys27-Pro220.[Accession | P16035]
Molecular Weight :
The protein has a predicted MW of 22.9 kDa. Due to glycosylation, the protein migrates to 24-26 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TIMP-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TIMP-2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Human TIMP-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-TIMP-2 Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.
Background :
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs).TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities.
Synonyms :
CSC-21K; TIMP2; DDC8; TIMP-2
References & Citations :
(1)Wang K , Wang G , Huang S , et al. Association between TIMP-2, gene polymorphism and breast cancer in Han Chinese women[J]. Bmc Cancer, 2019, 19(1):446.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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