Name : Human Adiponectin/Acrp30 Protein
Product Source :
Recombinant Human Adiponectin/Acrp30 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Glu19-Asn244.[Accession | Q15848]
Molecular Weight :
The protein has a predicted MW of 25.6 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Adiponectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Adiponectin is greater than 94% as determined by SEC-HPLC.
Background :
Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation.Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects.
Synonyms :
ACDC; Acrp30; ADPN; Adiponectin; AdipoQ; ADIPQTL1; APM1APM-1; GBP28; GBP28apM1; APM-1; APM1
References & Citations :
(1) Iwabu M , Yamauchi T , Okadaiwabu M , et al. [Adiponectin receptor-targeted therapy for lifestyle-related diseases].[J]. Clin Calcium, 2016, 26(3):413-418.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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