Name : Human TNFR1/CD120a/TNFRSF1A Protein
Product Source :
Recombinant Human TNFR1/CD120a/TNFRSF1A Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Leu30-Thr211.[Accession | P19438-1]
Molecular Weight :
The protein has a predicted MW of 23.3 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TNFR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TNFR1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human TNFR1, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 16.5ng/ml determined by ELISA.
Background :
Tumour necrosis factor alpha (TNF-α) is a pleiotropic cytokine with both injurious and protective functions, which are thought to diverge at the level of its two cell surface receptors, TNFR1 and TNFR2. In the setting of acute injury, selective inhibition of TNFR1 is predicted to attenuate the cell death and inflammation associated with TNF-α, while sparing or potentiating the protective effects of TNFR2 signalling.
Synonyms :
TNF-R1; TNF-RI; TNFR-I; TNFR1; CD120a; TNFRSF1A; FPF; TBP1; TNF-R; TNF-R55; TNFAR; TNFR60; p55; p60; MS5; p55-R; TNF-R-I; TNFR1-d2
References & Citations :
(1)Proudfoot A, Bayliffe A, O’Kane CM, Wright T, Serone A, Bareille PJ, Brown V, Hamid UI, Chen Y, Wilson R, Cordy J, Morley P, de Wildt R, Elborn S, Hind M, Chilvers ER, Griffiths M, Summers C, McAuley DF. Novel anti-tumour necrosis factor receptor-1 (TNFR1) domain antibody prevents pulmonary inflammation in experimental acute lung injury. Thorax. 2018 Aug;73(8):723-730. doi: 10.1136/thoraxjnl-2017-210305. Epub 2018 Jan 29. PMID: 29382797; PMCID: PMC6204954.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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