Name : Human tPA Protein
Product Source :
Recombinant Human tPA Protein is expressed from HEK293 with His tag at the C-terminus. It contains Ser36-Pro562, which consists of two chains: chain A (Ser36-Arg310) and chain B (Ile311-Pro562).[Accession | P00750-1]
Molecular Weight :
The protein has a predicted MW of 60.13 kDa. The protein is activated by thermolysin and cleaved to a 2-chain protease, it migrates to 40-45 kDa (Chain A) and 35-40 kDa (Chain B) based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 20mM MES, 300mM Nacl, 200mM Arginine (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 300mM Nacl, 200mM Arginine (pH 5.5).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human tPA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. Bioactivity Data Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is > 200 pmol/min/μg.
Background :
Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that thepredominant route for enhancing cell survival is via an mTORdependent mechanism.
Synonyms :
t-PA; tPA; Alteplase; Reteplase; PLAT;
References & Citations :
Grummisch JA, Jadavji NM, Smith PD. tPA promotes cortical neuron survival via mTOR-dependent mechanisms. Mol Cell Neurosci. 2016 Jul;74:25-33. doi: 10.1016/j.mcn.2016.03.005. Epub 2016 Mar 16. PMID: 26995507.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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