Name : Mouse TNFSF12/TWEAK Protein
Product Source :
Recombinant Mouse TNFSF12/TWEAK Protein is expressed from HEK293 with Rabbit Fc tag at the N-terminus. It contains Ser46-His249.[Accession | O54907]
Molecular Weight :
The protein has a predicted MW of 48.95 kDa. Due to furin cleavage and glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse TNFSF12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SPR Data Mouse TNFSF12, Rabbit Fc Tag immobilized on CM5 Chip can bind Mouse TNFRSF12A, hFc Tag with an affinity constant of 0.12 nM as determined in SPR assay (Biacore T200).
Background :
Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) is a well known multifunctional cytokine extensively distributed in cell types and tissues. Accumulating evidence has shown that TWEAK binding to the receptor factor-inducible 14 (Fn14) participates in diverse pathologic processes including cell proliferation and death, angiogenesis, carcinogenesis and inflammation.
Synonyms :
TNFSF12; APO3 ligand; APO3L; DR3LG; UNQ181/PRO207; TWEAK; MGC129581
References & Citations :
Wang M, Xie Z, Xu J, Feng Z. TWEAK/Fn14 axis in respiratory diseases. Clin Chim Acta. 2020 Oct;509:139-148. doi: 10.1016/j.cca.2020.06.007. Epub 2020 Jun 8. PMID: 32526219.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
RANTES/CCL5 ProteinSynonyms
BMP-4 ProteinBiological Activity
Popular categories:
IL-27 beta/EBI3
MRCK beta