Name : Biotinylated Human TRAIL R2/DR5/TNFRSF10B Protein
Product Source :
Recombinant Biotinylated Human TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ile56-Glu182.[Accession | O14763-1]
Molecular Weight :
The protein has a predicted MW of 17.2 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human TRAIL R2 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human TRAIL R2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human TRAIL R2, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-TRAIL R2 Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA.
Background :
DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.
Synonyms :
CD262; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRICK2; TRICK2A; TRICKB; ZTNFR9; KILLER
References & Citations :
(1)Zheng D , Liu Y. Is death receptor 4 or 5 a good traget for cancer antibody therapy?[J]. New Biotechnology, 2012, 29:S114.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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