Name : Mouse TRAIL R2/DR5/TNFRSF10B Protein
Product Source :
Recombinant Mouse TRAIL R2/DR5/TNFRSF10B Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asn53-Lys180.[Accession | Q9QZM4-1]
Molecular Weight :
The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by SEC-HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse TRAIL R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse TRAIL R2 is greater than 95% as determined by SEC-HPLC. SPR Data Mouse TRAIL R2, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.43 nM as determined in SPR assay (Biacore T200).
Background :
DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.
Synonyms :
CD262; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRICK2; TRICK2A; TRICKB; ZTNFR9; KILLER
References & Citations :
(1)Zheng D , Liu Y. Is death receptor 4 or 5 a good traget for cancer antibody therapy?[J]. New Biotechnology, 2012, 29:S114.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
EphA4 Proteinsite
DSG-2/Desmoglein-2 ProteinFormulation
Popular categories:
FcγRIIIA/CD16a
IL-24