Name : Human TREM2 Protein
Product Source :
Recombinant Human TREM2 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains His19-Ser174.[Accession | Q9NZC2-1]
Molecular Weight :
The protein has a predicted MW of 44.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TREM2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human TREM2, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-TREM2 Antibody, hFc Tag with the EC50 of 46.9ng/ml determined by ELISA (QC Test). SPR Data Human TREM2, hFc Tag captured on CM5 Chip via Protein A can bind Human APOE3, His Tag with an affinity constant of 1.21 μM as determined in SPR assay (Biacore T200).
Background :
TREM-2 (Triggering Receptor Expressed on Myeloid cells-2) is a 35 kDa type I transmembrane member of the TREM family and Ig superfamily. Mature human TREM-2 consists of a 156 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane (TM) domain, and a 35 aa cytoplasmic tail. TREM-2 forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia.
Synonyms :
PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c
References & Citations :
(1)Ford J W , Mcvicar D W. TREM and TREM-like receptors in inflammation and disease[J]. Current Opinion in Immunology, 2009, 21(1):38-46.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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