Name : Mouse BID Protein
Product Source :
Recombinant Mouse BID Protein is expressed from E.coli with His tag at the C-Terminus. It contains Ala1-Ser195.[Accession | EDK99650.1]
Molecular Weight :
The protein has a predicted MW of 22.72 kDa same as Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 90% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse BID on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse BID is greater than 90% as determined by SEC-HPLC. SPR Data Mouse BID, His Tag immobilized on CM5 Chip can bind Human BCL2L1, His Tag with an affinity constant of 33.06 nM as determined in SPR assay (Biacore T200).
Background :
Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.
Synonyms :
p22 BID; BID;
References & Citations :
Yin XM. Bid, a BH3-only multi-functional molecule, is at the cross road of life and death. Gene. 2006 Mar 15;369:7-19. doi: 10.1016/j.gene.2005.10.038. Epub 2006 Jan 27. PMID: 16446060.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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