Name : Biotinylated Human TSLP (R127A, R130A) Protein
Product Source :
Recombinant Biotinylated Human TSLP (R127A, R130A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Tyr29-Gln159(R127A,R130A).[Accession | Q969D9-1]
Molecular Weight :
The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human TSLP (R127A, R130A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human TSLP (R127A, R130A), His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Anti-TSLP Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TSLP (R127A, R130A) , His Tag with the EC50 of 3.2ng/ml determined by ELISA.
Background :
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Synonyms :
Thymic stromal lymphopoietin; TSLP
References & Citations :
(1)Corren J , Parnes J R , Wang L , et al. Tezepelumab in Adults with Uncontrolled Asthma[J]. New England Journal of Medicine, 2017, 377(10):936-946.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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