Name : Human TSLPR Protein
Product Source :
Recombinant Human TSLPR Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gly25-Lys231.[Accession | Q9HC73-1]
Molecular Weight :
The protein has a predicted MW of 50.8 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human TSLPR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human TSLPR is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human TSLPR, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-TSLPR Antibody, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test). Immobilized Human TSLP (R127A, R130A) , His Tag at 5μg/ml (100μl/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA (QC Test).
Background :
Thymic stromal lymphopoietin (TSLP) is an interleukin-7 (IL-7) like cytokine, which plays an important role in the regulation of immune responses to allergens. TSLP binds to a heterodimeric receptor complex composed of the IL-7 receptor alpha chain (IL-7Ralpha) and the TSLP receptor (TSLPR, also known as CRLF2).
Synonyms :
TSLP receptor; TSLPR; CRL2; CRLF2Y; CRLF2; IL-XR; p2RY8/CRLF2 fusion; CRLF2
References & Citations :
(1)Blagoev B, Nielsen MM, Angrist M, Chakravarti A, Pandey A. Cloning of rat thymic stromal lymphopoietin receptor (TSLPR) and characterization of genomic structure of murine Tslpr gene. Gene. 2002 Feb 6;284(1-2):161-8. doi: 10.1016/s0378-1119(02)00380-3. PMID: 11891057.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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