Name : Human ULBP-4 Protein
Product Source :
Recombinant Human ULBP-4 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains His31-Asp225.[Accession | Q8TD07-1]
Molecular Weight :
The protein has a predicted MW of 49.12 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ULBP-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ULBP-4 is greater than 95% as determined by SEC-HPLC. SPR Data Human ULBP-4, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 0.17 μM as determined in SPR assay (Biacore T200).
Background :
UL16-binding proteins (ULBPs) are markers of cellular stress which are upregulated on the surface of virus-infected and tumor cells. ULBPs are expressed by a variety of leukemias, carcinomas, melanomas, and tumor cell lines.ULBP4 is associated with higher levels of CD8+/NKG2D+ lymphocytes infiltrating the carcinoma and is a prognosticator of improved survival.
Synonyms :
N2DL-4; NKG2DL4; RAE-1-like transcript 4; RAET1E; LETAL; N2DL4; ULBP4;
References & Citations :
Sutherland CL, Rabinovich B, Chalupny NJ, Brawand P, Miller R, Cosman D. ULBPs, human ligands of the NKG2D receptor, stimulate tumor immunity with enhancement by IL-15. Blood. 2006 Aug 15;108(4):1313-9. doi: 10.1182/blood-2005-11-011320. Epub 2006 Apr 18. PMID: 16621962.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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