Name : Biotinylated Human ULBP-2 Protein
Product Source :
Recombinant Biotinylated Human ULBP-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Gly26-Ser217.[Accession | Q9BZM5]
Molecular Weight :
The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human ULBP-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human ULBP-2 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Biotinylated Human ULBP-2, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.25μg/ml determined by ELISA. Blocking Data Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.
Background :
ULBP2 expression was however linked to expression of mature CD57() NK cells. In particular, expression of ULBP2 was strongest on those NK cells that had evidence of recent activation and proliferation. We suggest that ULBP2 could be used to identify recently activated “mature” NK cells.
Synonyms :
ALCAN-alpha; N2DL2; NKG2DL2; RAET1H; RAET1L; ULBP2; ULBP-2/5/6; NKG2D ligand 2
References & Citations :
(1) Brennan K, McSharry BP, Keating S, Petrasca A, O’Reilly VP, Keane J, Doherty DG, Gardiner CM. Human Natural Killer cell expression of ULBP2 is associated with a mature functional phenotype. Hum Immunol. 2016 Oct;77(10):876-885. doi: 10.1016/j.humimm.2016.06.018. Epub 2016 Jun 24. PMID: 27349945.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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