Name : Mouse VCAM-1/CD106 Protein

Product Source :
Recombinant Mouse VCAM-1/CD106 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Phe25-Glu698.[Accession | NP_035823.3]

Molecular Weight :
The protein has a predicted MW of 75.48 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse VCAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse VCAM-1 is greater than 95% as determined by SEC-HPLC. Bioactivity Data Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to Mouse VCAM-1 coated plates (10 μg/ml with 100 μl/well) , approximately 70 % – 80 % cells will adhere after 1 hour incubation at room temperature.

Background :
Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that triggers the expression of inflammatory molecules, including other cytokines and cell adhesion molecules. TNFα induces the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 was originally identified as a cell adhesion molecule that helps regulate inflammation-associated vascular adhesion and the transendothelial migration of leukocytes, such as macrophages and T cells.

Synonyms :
V-CAM 1; VCAM-1; INCAM-100; CD106; DKFZp779G2333; MGC99561

References & Citations :
(1) Kong DH, Kim YK, Kim MR, Jang JH, Lee S. Emerging Roles of Vascular Cell Adhesion Molecule-1 (VCAM-1) in Immunological Disorders and Cancer. Int J Mol Sci. 2018 Apr 2;19(4):1057. doi: 10.3390/ijms19041057. PMID: 29614819; PMCID: PMC5979609.

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