Name : Biotinylated Human VEGF-C/Flt4-L Protein
Product Source :
Recombinant Biotinylated Human VEGF-C/Flt4-L Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Thr103-Arg227.[Accession | Q6FH59]
Molecular Weight :
The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 6.0).
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human VEGF-C on Tris-Bis PAGE under reduced (R) condition. The purity is greater than 95%. ELISA Data Immobilized Biotinylated Human VEGF-C, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.4ng/ml determined by ELISA.
Background :
the lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes.
Synonyms :
VEGFC; VEGFc; Flt4-L; VRP; VEGF-C
References & Citations :
(1)Lim L, Bui H, Farrelly O, Yang J, Li L, Enis D, Ma W, Chen M, Oliver G, Welsh JD, Kahn ML. Hemostasis stimulates lymphangiogenesis through release and activation of VEGFC. Blood. 2019 Nov 14;134(20):1764-1775. doi: 10.1182/blood.2019001736. PMID: 31562136; PMCID: PMC6856989.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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