Name : Human Vitronectin Protein
Product Source :
Recombinant Human Vitronectin Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Asp20-Leu478.[Accession | P04004]
Molecular Weight :
The protein has a predicted MW of 53.37 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Vitronectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human Vitronectin is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human Vitronectin, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 1.04μg/ml determined by ELISA.
Background :
Vitronectin is a multifunctional glycoprotein present in blood and in the extracellular matrix. It binds glycosaminoglycans, collagen, plasminogen and the urokinase-receptor, and also stabilizes the inhibitory conformation of plasminogen activation inhibitor-1.
Synonyms :
VN; S-protein; Serum-spreading factor; V75; VTN
References & Citations :
Schvartz I, Seger D, Shaltiel S. Vitronectin. Int J Biochem Cell Biol. 1999 May;31(5):539-44. doi: 10.1016/s1357-2725(99)00005-9. PMID: 10399314.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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