Name : Human Wnt Surrogate-Fc Fusion Protein
Product Source :
Recombinant Human Wnt Surrogate-Fc Fusion Protein is expressed from HEK293 with hFc tag at the C-Terminus.
Molecular Weight :
The protein has a predicted MW of 58.50 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human Wnt Surrogate-Fc Fusion on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. Cell Based Assay Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 5.2 ng/mL.
Background :
Wnt-3a is regarded as an activator of the canonical Wnt signaling pathway. This activator is expressed in the dorsal midline region and is responsible for spinal cord development. In addition, Wnt-3a plays a regulatory role in autophagy, apoptosis, and regeneration of neurons, neurogenic inflammation and axon regeneration.Wnt-3a promotes the beta-catenin/Tcf pathway which is tumor inducing and can cause cancer when expressed in particular cell populations. It is also one of the most commonly used cytokines for organoid construction.
Synonyms :
Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a
References & Citations :
[1] Gao K, Zhang T, Wang F, Lv C. Therapeutic Potential of Wnt-3a in Neurological Recovery after Spinal Cord Injury. Eur Neurol. 2019;81(3-4):197-204. doi: 10.1159/000502004. Epub 2019 Jul 23. PMID: 31336380.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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