Name : Mouse VSTM5 Protein
Product Source :
Recombinant Mouse VSTM5 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu28-His146.[Accession | Q9D806]
Molecular Weight :
The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse VSTM5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse VSTM5 is greater than 95% as determined by SEC-HPLC.
Background :
V-set and transmembrane domain-containing protein 5 (Vstm5), a cell-adhesion-like molecule belonging to the Ig superfamily, was found in mouse brain. Knock-down of Vstm5 in cultured hippocampal neurons markedly reduced the complexity of dendritic structures, as well as the number of dendritic filopodia. Vstm5 also regulates neuronal morphology by promoting dendritic protrusions that later develop into dendritic spines.
Synonyms :
C11orf90; VSTM5
References & Citations :
(1) Lee AR, Ko KW, Lee H, Yoon YS, Song MR, Park CS. Putative Cell Adhesion Membrane Protein Vstm5 Regulates Neuronal Morphology and Migration in the Central Nervous System. J Neurosci. 2016 Sep 28;36(39):10181-97. doi: 10.1523/JNEUROSCI.0541-16.2016. Epub 2016 Sep 28. PMID: 27683913; PMCID: PMC6705580.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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