Name : Biotinylated Human Serum Albumin Protein
Product Source :
Recombinant Biotinylated Human Serum Albumin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Asp25-Leu609.[Accession | P02768-1]
Molecular Weight :
The protein has a predicted MW of 69.4 kDa. Due to glycosylation, the protein migrates to 69-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human Serum Albumin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human Serum Albumin is greater than 95% as determined by SEC-HPLC. SPR Data Biotinylated Human Serum Albumin, His-Avi Tag immobilized on CM5 Chip can bind Human FcRn, His Tag with an affinity constant of 1.67 μM as determined in SPR assay (Biacore T200).
Background :
Human serum albumin (HSA), the most prominent protein in plasma, binds different classes of ligands at multiple sites. HSA provides a depot for many compounds, affects pharmacokinetics of many drugs, holds some ligands in a strained orientation providing their metabolic modification, renders potential toxins harmless transporting them to disposal sites, accounts for most of the antioxidant capacity of human serum, and acts as a NO-carrier.
Synonyms :
Albumin; ALB; Serum albumin; ANALBA; FDAH; PRO0883; PRO0903; PRO1341
References & Citations :
(1) Ishima Y, Maruyama T. [Human Serum Albumin as Carrier in Drug Delivery Systems]. Yakugaku Zasshi. 2016;136(1):39-47. Japanese. doi: 10.1248/yakushi.15-00227-1. PMID: 26725666.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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