Name : Human YKL-40/CHI3L1 Protein
Product Source :
Recombinant Human YKL-40/CHI3L1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Tyr22-Thr383.[Accession | P36222]
Molecular Weight :
The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 42-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human YKL-40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human YKL-40 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human YKL-40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-YKL-40 Antibody, hFc Tag with the EC50 of 17.8ng/ml determined by ELISA.
Background :
YKL-40 (also known as Chitinase 3-like 1) is a glycoprotein produced by inflammatory, cancer and stem cells. Its physiological role is not completely understood but YKL-40 is elevated in the brain and cerebrospinal fluid (CSF) in several neurological and neurodegenerative diseases associated with inflammatory processes. Yet the precise characterization of YKL-40 in dementia cases is missing. YKL-40 is a disease-specific marker of neuroinflammation showing its highest levels in prion diseases. Therefore, YKL-40 quantification might have a potential for application in the evaluation of therapeutic intervention in dementias with a neuroinflammatory component.
Synonyms :
BRP39; CGP-39; GP-39; Chil1; ASRT7; FLJ38139; HC-gp39; YKL-40; YKL40; YYL-40; AW208766; CHI3L1; Chitinase 3 like 1
References & Citations :
(1) Llorens F, Thüne K, Tahir W, Kanata E, Diaz-Lucena D, Xanthopoulos K, Kovatsi E, Pleschka C, Garcia-Esparcia P, Schmitz M, Ozbay D, Correia S, Correia Â, Milosevic I, Andréoletti O, Fernández-Borges N, Vorberg IM, Glatzel M, Sklaviadis T, Torres JM, Krasemann S, Sánchez-Valle R, Ferrer I, Zerr I. YKL-40 in the brain and cerebrospinal fluid of neurodegenerative dementias. Mol Neurodegener. 2017 Nov 10;12(1):83. doi: 10.1186/s13024-017-0226-4. PMID: 29126445; PMCID: PMC5681777.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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