Name : Human BTN1A1/Butyrophilin Protein

Product Source :
Recombinant Human BTN1A1/Butyrophilin Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ala27-Arg242.[Accession | Q13410]

Molecular Weight :
The protein has a predicted MW of 26.9 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human BTN1A1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human BTN1A1 is greater than 95% as determined by SEC-HPLC.

Background :
Butyrophilin 1A1 (BTN1A1) is one of the membrane proteins that surrounds LD,BTN1A1 plays an important role in regulating LD synthesis via a mechanism involving membrane phospholipid composition.

Synonyms :
BT; BTN1A1; BTN; Butyrophilin

References & Citations :
(1) Han L, Zhang M, Xing Z, Coleman DN, Liang Y, Loor JJ, Yang G. Knockout of butyrophilin subfamily 1 member A1 (BTN1A1) alters lipid droplet formation and phospholipid composition in bovine mammary epithelial cells. J Anim Sci Biotechnol. 2020 Jul 3;11:72. doi: 10.1186/s40104-020-00479-6. PM

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