Name : Human ADAM8 Protein
Product Source :
Recombinant Human ADAM8 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ile17-Pro655.[Accession | P78325]
Molecular Weight :
The protein has a predicted MW of 70.9 kDa. The protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human ADAM8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human ADAM8 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human ADAM8, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-ADAM8 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. Bioactivity Data Measured by its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2. The specific activity is >1 pmol/min/µg
Background :
A Disintegrin And Metalloproteinase (ADAM) proteases constitute a family of multifunctional, membrane-bound proteins with traditional sheddase functions. Their protumorigenic potential has been attributed to both, essential (ADAM10 and ADAM17) and ‘dispensable’ ADAM proteases (ADAM8, 9, 12, 15, and 19). Of specific interest in this review is the ADAM proteinase ADAM8 that has been identified as a significant player in aggressive malignancies including breast, pancreatic, and brain cancer.
Synonyms :
ADAM 8; Ms2; Adam8; CD156a; MGC134985
References & Citations :
(1) Conrad C, Benzel J, Dorzweiler K, Cook L, Schlomann U, Zarbock A, Slater EP, Nimsky C, Bartsch JW. ADAM8 in invasive cancers: links to tumor progression, metastasis, and chemoresistance. Clin Sci (Lond). 2019 Jan 11;133(1):83-99. doi: 10.1042/CS20180906. PMID: 30635388.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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