Name : Human CA9/Carbonic Anhydrase IX Protein
Product Source :
Recombinant Human CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with hFc tag at the C-terminus. It contains Gln38-Asp414.[Accession | Q16790]
Molecular Weight :
The protein has a predicted MW of 66.91 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution :
Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CA9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CA9 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CA9, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CA9 Antibody, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA. Bioactivity Data Measured by its esterase activity. The specific activity is > 50 pmol/min/µg.
Background :
CA9 is a member of the Carbonic Anhydrases’ family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.
Synonyms :
CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC
References & Citations :
(1)Potter C , Harris A L. Hypoxia Inducible Carbonic Anhydrase IX, Marker of Tumour: Hypoxia, Survival Pathway and Therapy Target[J]. Cell Cycle, 2004, 3(2):159-162.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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