Name : Mouse CA9/Carbonic Anhydrase IX Protein
Product Source :
Recombinant Mouse CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln32-Asp390.[Accession | NP_647466.2]
Molecular Weight :
The protein has a predicted MW of 43.12 kDa. Due to glycosylation, the protein migrates to 50-53 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per ug by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse CA9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse CA9 is greater than 95% as determined by SEC-HPLC.
Background :
CA9 is a member of the Carbonic Anhydrases’ family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.
Synonyms :
CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC
References & Citations :
(1)Potter C , Harris A L. Hypoxia Inducible Carbonic Anhydrase IX, Marker of Tumour: Hypoxia, Survival Pathway and Therapy Target[J]. Cell Cycle, 2004, 3(2):159-162.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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