Name : Human EpCAM/TROP1 Protein
Product Source :
Recombinant Human EpCAM/TROP1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln24-Lys265.[Accession | P16422]
Molecular Weight :
The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human EpCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.. SEC-HPLC The purity of Human EpCAM is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human EpCAM, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA.
Background :
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Synonyms :
CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; ACSTD1; DIAR5; gp40; HNPCC8; MOC31; TACST-1; TACSTD1; TROP1; ESA; KSA; MIC18; MK1
References & Citations :
(1)Pak M G , Shin D H , Lee C H , et al. Significance of EpCAM and TROP2 expression in non-small cell lung cancer[J]. World Journal of Surgical Oncology, 2012, 10(1):53
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
Popular product recommendations:
PLA2G2D ProteinStorage & Stability
Alpha-2-Antiplasmin/Serpin F2 ProteinFormulation
Popular categories:
IL-27 beta/EBI3
Lymphotoxin-β