Name : Human AREG Protein
Product Source :
Recombinant Human AREG Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Ser101-Lys187.[Accession | P15514]
Molecular Weight :
The protein has a predicted MW of 37.3 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human AREG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human AREG is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human AREG, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-AREG Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA.
Background :
Amphiregulin (AREG) is a member of the epidermal growth factor (EGF) family and is expressed in a plethora of cancers. Tumour growth and metastasis were decreased by AREG silencing in an orthotopic model of pancreatic cancer. AREG may play a critical role in cell migration, invasion, and EMT by activating the EGFR/ERK/NF‑κB signalling pathway in pancreatic cancer cells.
Synonyms :
Amphiregulin; AR; SDGF; AREG; AREGB; CRDGF; MGC13647
References & Citations :
(1) Wang L, Wang L, Zhang H, Lu J, Zhang Z, Wu H, Liang Z. AREG mediates the epithelial‑mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF‑κB signalling pathway. Oncol Rep. 2020 May;43(5):1558-1568. doi: 10.3892/or.2020.7523. Epub 2020 Feb 27. PMID: 32323797; PMCID: PMC7107775.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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