Name : Human CCR7 Protein-Nanodisc

Product Source :
Recombinant Human CCR7 Protein-Nanodisc is expressed from HEK293 with His tag at the C-terminus. It contains Met1-Pro378.[Accession | P32248]

Molecular Weight :
The protein has a predicted MW of 67.70 kDa.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :

Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
ELISA Data Immobilized Human CCR7 (Nanodisc), His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-CCR7 Antibody, hFc Tag with the EC50 of 0.21μg/ml determined by ELISA (QC Test). SPR Data Human CCR7 (Nanodisc), His Tag captured on CM5 Chip via Anti-his antibody can bind Anti-CCR7 Antibody, hFc Tag with an affinity constant of 4.11 nM as determined in SPR assay (Biacore T200).

Background :
CC-chemokine receptor 7 (CCR7), collaborated with its ligands CCL19 and CCL21, controls extensive migratory events in the immune system. CCR7-bearing dendritic cells can swarm into T-cell zones in lymph nodes, initiating the antigen presentation and T-cell response. Abnormal expression of CCR7 in dendritic cells will cause a series of inflammatory diseases due to the chaotic dendritic cell trafficking.

Synonyms :
CCR7; C-C CKR-7; CC-CKR-7; CCR-7; BLR2; CDw197; EBI1; CD197; CMKBR7; EBI1; EVI1

References & Citations :
Hong W, Yang B, He Q, Wang J, Weng Q. New Insights of CCR7 Signaling in Dendritic Cell Migration and Inflammatory Diseases. Front Pharmacol. 2022 Feb 25;13:841687. doi: 10.3389/fphar.2022.841687. PMID: 35281921; PMCID: PMC8914285.

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