Name : Mouse AREG Protein
Product Source :
Recombinant Mouse AREG Protein is expressed from HEK293 with hFc tag at the N-Terminus. It contains Val100-Ala248.[Accession | P31955]
Molecular Weight :
The protein has a predicted MW of 38.6 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Mouse AREG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse AREG is greater than 95% as determined by SEC-HPLC.
Background :
Amphiregulin (AREG) is a ligand of the epidermal growth factor receptor (EGFR), a widely expressed transmembrane tyrosine kinase. AREG is synthesized as a membrane-anchored precursor protein that can engage in juxtacrine signaling on adjacent cells. Alternatively, after proteolytic processing by cell membrane proteases, mainly TACE/ADAM17, AREG is secreted and behaves as an autocrine or paracrine factor.
Synonyms :
Amphiregulin; AR; SDGF; AREG; AREGB; CRDGF; MGC13647
References & Citations :
(1) Berasain C, Avila MA. Amphiregulin. Semin Cell Dev Biol. 2014 Apr;28:31-41. doi: 10.1016/j.semcdb.2014.01.005. Epub 2014 Jan 23. PMID: 24463227.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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