Name : Human CD14 Protein
Product Source :
Recombinant Human CD14 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Thr20-Met344.[Accession | AAH10507]
Molecular Weight :
The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD14 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD14 is greater than 95% as determined by SEC-HPLC.
Background :
Human monocyte differentiation antigen CD14 is a pattern recognition receptor (PRR) that enhances innate immune responses. CD14 was first identified as a marker of monocytes to signal intracellular responses upon bacterial encounters. Given the absence of an intracellular tail, CD14 was doubted to have the signaling capacities.
References & Citations :
(1) Wu Z, Zhang Z, Lei Z, Lei P. CD14: Biology and role in the pathogenesis of disease. Cytokine Growth Factor Rev. 2019 Aug;48:24-31. doi: 10.1016/j.cytogfr.2019.06.003. Epub 2019 Jul 3. PMID: 31296363.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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