Name : Cynomolgus/Rhesus macaque CD27/TNFRSF7 Protein
Product Source :
Recombinant Cynomolgus/Rhesus macaque CD27/TNFRSF7 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala20-Arg191.[Accession | XP_005569963.1]
Molecular Weight :
The protein has a predicted MW of 46.5 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus/Rhesus macaque CD27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus/Rhesus macaque CD27 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus CD27 Ligand, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus/Rhesus macaque CD27, hFc Tag with the EC50 of 41.3ng/ml determined by ELISA.
Background :
CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
Synonyms :
CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2; MGC20393; S152CD27L receptor
References & Citations :
(1)Buchan S L , Rogel A , Al-Shamkhani A. The immunobiology of CD27 and OX40 and their potential as targets for cancer immunotherapy[J]. Blood, 2017:blood-2017-07-741025.
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