Name : Human CD2/SRBC Protein
Product Source :
Recombinant Human CD2/SRBC Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Lys25-Asp209.[Accession | P06729]
Molecular Weight :
The protein has a predicted MW of 22.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by SEC-HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD2 is greater than 95% as determined by SEC-HPLC. SPR Data Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200).
Background :
The CD2 family of receptors is evolutionarily conserved and widely expressed on cells within the hematopoietic compartment. In recent years several new members have been identified with important roles in the immune system. CD2 family members regulate natural killer (NK) cell lytic activity and inflammatory cytokine production when engaged by ligands on tumor cells.
Synonyms :
T11; CD2; SRBC; LFA-2; LFA-3 receptor
References & Citations :
(1)Shao T, Shi W, Zheng JY, Xu XX, Lin AF, Xiang LX, Shao JZ. Costimulatory Function of Cd58/CD2 Interaction in Adaptive Humoral Immunity in a Zebrafish Model. Front Immunol. 2018 May 31;9:1204. doi: 10.3389/fimmu.2018.01204. PMID: 29904386; PMCID: PMC5990624.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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