Name : Human CD200/OX-2 Protein
Product Source :
Recombinant Human CD200/OX-2 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Gln31-Gly232.[Accession | P41217-1]
Molecular Weight :
The protein has a predicted MW of 23.5 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD200 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD200 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD200, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 21.6ng/ml determined by ELISA.
Background :
CD200 and its receptors are highly expressed in the lung, on epithelial cells and leukocytes, and emerging evidence links dysregulation of the CD200 pathway with asthma. Moreover, pharmacological modulation of CD200 receptors was shown to improve clinical and inflammatory outcomes of preclinical asthma models.
Synonyms :
CD200; MOX1; MOX2; My033; MRC; OX-2; OX2
References & Citations :
(1)Ngwa C, Liu F. CD200-CD200R signaling and diseases: a potential therapeutic target? Int J Physiol Pathophysiol Pharmacol. 2019 Dec 15;11(6):297-309. PMID: 31993106; PMCID: PMC6971504.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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