Name : Mouse CD200 R1 Protein
Product Source :
Recombinant Mouse CD200 R1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Thr26-Pro238.[Accession | Q9ES57]
Molecular Weight :
The protein has a predicted MW of 24.21 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Mouse CD200 R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. The purity of Mouse CD200 R1 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse CD200R1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 0.53μg/ml determined by ELISA (QC Test). SPR Data Mouse CD200 R1, His Tag immobilized on CM5 Chip can bind Mouse CD200, His Tag with an affinity constant of 1.19 μM as determined in SPR assay (Biacore T200).
Background :
CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.
Synonyms :
CD200R; CRTR2; MOX2R; OX2R; CD200 R1; CD200 receptor 1; HCRTR2; MOX2 receptor
References & Citations :
(1)Gorczynski R, Chen Z, Kai Y, Lee L, Wong S, Marsden PA. CD200 is a ligand for all members of the CD200R family of immunoregulatory molecules. J Immunol. 2004;172(12):7744-7749. doi:10.4049/jimmunol.172.12.7744.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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