Name : Mouse CD27/TNFRSF7 Protein

Product Source :
Recombinant Mouse CD27/TNFRSF7 Protein is expressed from HEK293 with His tag at the C-terminus. It contains Pro24-Arg182.[Accession | P41272]

Molecular Weight :
The protein has a predicted MW of 19.40 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC

Formulation :
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).

Reconstitution :

Storage and Stability :
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Mouse CD27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Mouse CD27 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Mouse CD27, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.

Background :
CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.

Synonyms :
CD27 antigen; Tp55; TNFRSF7; S152; T14; S152. LPFS2

References & Citations :
(1)Buchan S L , Rogel A , Al-Shamkhani A. The immunobiology of CD27 and OX40 and their potential as targets for cancer immunotherapy[J]. Blood, 2017:blood-2017-07-741025.

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