Name : Cynomolgus CD31/PECAM-1 Protein

Product Source :
Recombinant Cynomolgus CD31/PECAM-1 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains His28-Gly602.[Accession | A0A7N9CHZ3]

Molecular Weight :
The protein has a predicted MW of 65.30 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Cynomolgus CD31 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Background :
Platelet endothelial cell adhesion molecule 1 (PECAM-1) is an adhesion and signaling receptor that is expressed on endothelial and hematopoietic cells and plays important roles in angiogenesis, vascular permeability, and regulation of cellular responsiveness.

Synonyms :
PECAM-1; EndoCAM; PECA1; CD31; FLJ34100; FLJ58394; GPIIA’

References & Citations :
(1) Zhi H, Kanaji T, Fu G, Newman DK, Newman PJ. Generation of PECAM-1 (CD31) conditional knockout mice. Genesis. 2020 Feb;58(2):e23346. doi: 10.1002/dvg.23346. Epub 2019 Nov 15. PMID: 31729819; PMCID: PMC7021573.

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