Name : Cynomolgus CD3e&CD3G/CD3 epsilon&CD3 gamma Protein
Product Source :
Recombinant Cynomolgus CD3e&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Gln22-Asp117(CD3e)&Gln23-Thr113(CD3G).[Accession | Q95LI5.2(CD3e)&Q95LI7(CD3G)]
Molecular Weight :
The protein has a predicted MW of 36.9 kDa (CD3e) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Cynomolgus CD3e&CD3G on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. SEC-HPLC The purity of Cynomolgus CD3e&CD3G was greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Cynomolgus CD3e&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with EC50 of 20.1ng/ml determined by ELISA.
Background :
T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3e & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3e, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Synonyms :
CD3; CD3G; CD3g; T3G; CD3e; CD3e; CD3 epsilon&CD3 gamma; CD3e&CD3G
References & Citations :
(1)Penaranda C , Tang Q , Bluestone J A. Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022.
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