Name : Human CD3E&CD3d/CD3 epsilon&CD3 delta Protein
Product Source :
Recombinant Human CD3E&CD3d/CD3 epsilon&CD3 delta Protein is expressed from HEK293 with hFc at the C-Terminus. It contains Asp23-Asp126(CD3E)&Phe22-Ala105(CD3d).[Accession | P07766(CD3E)&P04234(CD3d)]
Molecular Weight :
The protein has a predicted MW of 37.8 kDa (CD3E) and 35.4 kDa (CD3d). Due to glycosylation, the protein migrates to 48-58 kDa (CD3E) and 42-48 kDa (CD3d) based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD3E&CD3d on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD3E&CD3d was greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD3E&CD3d, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3d Ab.1, hFc Tag with the EC50 of 31.68ng/ml determined by ELISA. ELISA Data Immobilized Human CD3E&CD3d, hFc Tag at 1μg/ml (100ul/Well). Dose response curve for Anti-CD3E&CD3d Ab.2, mFc Tag with the EC50 of 45.9ng/ml determined by ELISA (QC Test). ELISA Data Immobilized Tebentafusp, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human CD3E&CD3d, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.
Background :
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3d , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3d, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Synonyms :
CD3; CD3e; CD3E; CD3d; T3D; CD3d; CD3E&CD3d; CD3 delta&CD3 epsilon
References & Citations :
(1)Penaranda C , Tang Q , Bluestone J A. Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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