Name : Biotinylated Human CD3e/CD3 epsilon 1-27 Protein
Product Source :
Recombinant Biotinylated Human CD3e/CD3 epsilon 1-27 Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-Terminus. It contains Asp23-Thr48.[Accession | P07766]
Molecular Weight :
The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human CD3e 1-27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human CD3e 1-27 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Anti-CD3 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD3e 1-27, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Background :
CD3e, is a single-pass type I membrane protein.CD3 (cluster of differentiation 3) T cell co-receptor helps to activate both the cytotoxic T cell (CD8 naive T cells) and also T helper cells (CD4 naive T cells). It consists of a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains.
Synonyms :
CD3e; CD3e; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
References & Citations :
(1)Kroesen B J , Nieken J , Sleijfer D T , et al. Approaches to lung cancer treatment using the CD3e×GP-2-directed Bispecific Monoclonal Antibody BIS-1[J]. Cancer Immunology Immunotherapy, 1997, 45(3-4):203-206.
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