Name : Human CD3e/CD3 epsilon Protein
Product Source :
Recombinant Human CD3e/CD3 epsilon Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Asp23-Asp126(C119S, C122S).[Accession | P07766]
Molecular Weight :
The protein has a predicted MW of 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD3e on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. ELISA Data Immobilized Human CD3e, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 33.5ng/ml determined by ELISA (QC Test). Batch to Batch Consistency Immobilized Human CD3e, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 33.5/36.2/35.2ng/ml determined by ELISA.
Background :
T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3e & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3e, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Synonyms :
CD3e; CD3e; T3E; FLJ18683; TCRE; CD3-epsilon; IMD18
References & Citations :
(1)Penaranda C , Tang Q , Bluestone J A. Anti-CD3 Therapy Promotes Tolerance by Selectively Depleting Pathogenic Cells while Preserving Regulatory T Cells[J]. The Journal of Immunology, 2011, 187(4):2015-2022.D
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