Name : Human CD43 Protein

Product Source :
Recombinant Human CD43 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Ser20-Arg253.[Accession | P16150]

Molecular Weight :
The protein has a predicted MW of 24.5 kDa. Due to glycosylation, the protein migrates to 75-120 kDa based on Tris-Bis PAGE result.

Endotoxin Level :
Less than 1EU per μg by the LAL method.

Purity :
> 95% as determined by Tris-Bis PAGE

Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Product Concentration :
Tris-Bis PAGE Human CD43 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Background :
CD43 (leukosialin) is a large sialoglycoprotein abundantly expressed on the surface of most cells from the hematopoietic lineage. CD43 is directly involved in the contact between cells participating in a series of events such as signaling, adherence and host parasite interactions. The induction of acute myocarditis involves the engagement of CD43 cytoplasmic tripeptide sequence KRR to ezrin-radixin-moiesin cytoskeletal proteins.

Synonyms :
Leukosialin; CD43; Ly-48; A630014B01Rik; Galgp; GPL115; LSN; Sialophorin; SPN

References & Citations :
(1) Alisson-Silva F, Mantuano NR, Lopes AL, Vasconcelos-Dos-Santos A, Vale AM, Costa MM, Cannon JL, Oliveira AC, Todeschini AR. CD43 sialoglycoprotein modulates cardiac inflammation and murine susceptibility to Trypanosoma cruzi infection. Sci Rep. 2019 Jun 13;9(1):8628. doi: 10.1038/s41598-019-45138-7. PMID: 31197200; PMCID: PMC6565700.

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