Name : Biotinylated Human CD4/LEU3 Protein
Product Source :
Recombinant Biotinylated Human CD4/LEU3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Lys26-Trp390.[Accession | P01730]
Molecular Weight :
The protein has a predicted MW of 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Biotinylated Human CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Biotinylated Human CD4 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Anti-CD4 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD4, His Tag with the EC50 of 12.7ng/ml determined by ELISA.
Background :
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Synonyms :
CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
References & Citations :
(1)Lam S , Bollard C. T-cell therapies for HIV[J]. Immunotherapy, 2013, 5(4):407-414.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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