Name : Human CD4/LEU3 Protein
Product Source :
Recombinant Human CD4/LEU3 Protein is expressed from HEK293 with hFc at the C-Terminus. It contains Lys26-Trp390.[Accession | P01730]
Molecular Weight :
The protein has a predicted MW of 67.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD4 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD4 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD4 Antibody, hFc Tag with the EC50 of 24.8ng/ml determined by ELISA.
Background :
CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.
Synonyms :
CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3
References & Citations :
(1) Lam S , Bollard C. T-cell therapies for HIV[J]. Immunotherapy, 2013, 5(4):407-414.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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