Name : Human CD59 Protein
Product Source :
Recombinant Human CD59 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Leu26-Asn102.[Accession | P13987-1]
Molecular Weight :
The protein has a predicted MW of 35.73 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3-6 months after reconstitution.2-8°C for 2-7 days after reconstitution.Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD59 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD59 is greater than 95% as determined by SEC-HPLC.
Background :
CD59 regulates complement activation cascade at the final step, inhibiting formation of membrane attack complex (MAC). This protein, being anchored to the cell membrane via glycosyl phosphatidyl inositol (GPI), is expressed ubiquitously on cells which are in contact with body fluids containing components.
Synonyms :
CD59; 1F5; HRF-20; HRF20; MAC-IP; MACIP; MEM43; MACIF; MIRL; G344; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21
References & Citations :
Sugita Y, Masuho Y. CD59: its role in complement regulation and potential for therapeutic use. Immunotechnology. 1995 Dec;1(3-4):157-68. doi: 10.1016/1380-2933(95)00018-6. PMID: 9373344.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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