Name : Human CD5 Protein
Product Source :
Recombinant Human CD5 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Arg25-Asn371.[Accession | P06127]
Molecular Weight :
The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.
Endotoxin Level :
Less than 1EU per μg by the LAL method.
Purity :
> 95% as determined by Tris-Bis PAGE> 95% as determined by HPLC
Formulation :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage and Stability :
-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Product Concentration :
Tris-Bis PAGE Human CD5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. SEC-HPLC The purity of Human CD5 is greater than 95% as determined by SEC-HPLC. ELISA Data Immobilized Human CD5 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 14.6ng/ml determined by ELISA.
Background :
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
References & Citations :
(1)Jevremovic, D., Dronca, R. S., Morice, W. G., McPhail, E. D., Kurtin, P. J., Zent, C. S., & Hanson, C. A. (2010). CD5 B-cell lymphoproliferative disorders: Beyond chronic lymphocytic leukemia and mantle cell lymphoma. Leukemia Research, 34(9), 1235-1238.
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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